origin 61 software  (OriginLab corp)

Journal: Cell stem cell

Article Title: Inhibition of PRC2 enables self-renewal of blastoid-competent naive pluripotent stem cells from chimpanzee.

doi: 10.1016/j.stem.2025.02.002

Figure Lengend Snippet: Figure 2. Transcriptome identity and trophectoderm differentiation of chimpanzee naive PSCs (A) Mapping of bulk RNA-seq samples for chimpanzee reset and primed PSCs onto UMAP embedding of human embryo scRNA-seq datasets.48 Left, embryo UMAP; center, projection of PSC samples; right, expanded ICM, hypoblast, and epiblast region. (B) Heatmap of correlation distance metrics between chimpanzee cell samples and human embryo cell types. Trophectoderm samples are from differentiation time points as in (G) below. (C) Heatmap of top 200 differentially expressed genes between naive and primed PSC samples in both chimpanzee and human. (D) Cluster map of pluripotency factor expression in human and chimpanzee naive or primed PSCs. (E) Immunostaining after treatment of naive PSCs with PD+A83 for 5 days. Scale bar: 278.5 mm.

Article Snippet: The aligned reads were processed by XenofilteR81 version 1.6 to remove transcripts of suspected mouse origin.61 10X Genomic single cell RNA sequencing of PSCs Samples were dissociated and labelled using the 3’ CellPlex kit Set A (10X Genomics 1000261), then multiplexed and single cells isolated using the Chromium Next Gem Chip G Single Cell kit (10X Genomics 1000120) on the Chromium X.

Techniques: RNA Sequencing, Expressing, Immunostaining

Journal: Cell stem cell

Article Title: Inhibition of PRC2 enables self-renewal of blastoid-competent naive pluripotent stem cells from chimpanzee.

doi: 10.1016/j.stem.2025.02.002

Figure Lengend Snippet: Figure 3. Chimpanzee naive PSCs form blastoids and can be generated by direct reprogramming (A) Phase images of day 4 blastoids from indicated lines. Scale bar: 139 mm. (B) Confocal images of blastoids immunostained for indicated lineage markers. Scale bar: 50 mm. (C) Numbers of hypoblast (FOXA2)- or epiblast (NANOG)-positive inner cells in individual day 4 blastoids. Double-negative cells may be trophoblast or nascent hypoblast. (D) UMAP with Leiden clustering of day 4 blastoid scRNA-seq data. (E) Projection of blastoid scRNA-seq samples on human embryo UMAP. (F) Comparison of blastoids and human embryo datasets by correlation distance metrics. Sample colors as in (D).

Article Snippet: The aligned reads were processed by XenofilteR81 version 1.6 to remove transcripts of suspected mouse origin.61 10X Genomic single cell RNA sequencing of PSCs Samples were dissociated and labelled using the 3’ CellPlex kit Set A (10X Genomics 1000261), then multiplexed and single cells isolated using the Chromium Next Gem Chip G Single Cell kit (10X Genomics 1000120) on the Chromium X.

Techniques: Generated, Comparison

Journal: iScience

Article Title: Capture and inactivation of viral particles from bioaerosols by electrostatic precipitation

doi: 10.1016/j.isci.2023.107567

Figure Lengend Snippet:

Article Snippet: Original CHO cell lines were obtained from ATCC (CCL-61).

Techniques: Virus, Recombinant, Transfection, Bicinchoninic Acid Protein Assay, SYBR Green Assay, Plasmid Preparation, Software, Real-time Polymerase Chain Reaction

Journal: The Journal of Biological Chemistry

Article Title: Rac1 GTPase regulates 11β hydroxysteroid dehydrogenase type 2 and fibrotic remodeling

doi: 10.1074/jbc.M116.764449

Figure Lengend Snippet: The RacET phenotype with age-dependent atrial dilatation, fibrosis, and atrial fibrillation. A and B, left atrial (LA) weight relative to tibia length (A) and to left ventricular (LV) weight (B) in transgenic mice with heart-specific overexpression of Rac1 at 6 (RacET 6) and 12 (RacET 12) months of age compared with WT control mice (FVBN). C and D, quantification of connective tissue growth factor (CTGF) mRNA (C, RT-PCR) and protein (D, Western blot) expression in LA of RacET mice. GAPDH was used as loading control. E and F, lysyl oxidase (E, LOX) and Sprouty-1 (F) myocardial protein expression in the RacET 6 and 12 months compared with WT mice. G, prevalence of atrial fibrillation and flutter evaluated by electrocardiography. H, Sirius red staining of left atrial myocardium of WT and 12-month-old RacET mice in ×10 (upper panel) and ×100 (lower panel) magnification. Error bars indicate means ± S.D. *, p < 0.05; **, p < 0.01; ***, p < 0.001.

Article Snippet: Immunoblotting was performed using anti-MR (rabbit polyclonal antibody against amino acids 1–300 of MR of human origin) (sc-11412, Santa Cruz), anti-11β-HSD2 (mouse monoclonal antibody against amino acids 261–405 of 11β-HSD2 of human origin) (sc-365529, Santa Cruz), anti-CTGF (goat polyclonal antibody against a peptide mapping within an internal region of CTGF of human origin) (sc-14939, Santa Cruz), anti-Sprouty-1 (rabbit polyclonal antibody against amino acids 61–180 mapping within an internal region of Sprouty-1 of human origin) (sc-30048, Santa Cruz), anti-LOX (rabbit polyclonal antibody against residues 350 to the C terminus of human LOX) (ab31238, Abcam), anti-Rac1 (mouse IgG 2b against recombinant protein containing the full-length human Rac1, Clone 23A8) (05–389, EMD Millipore) and anti-fibronectin (mouse monoclonal antibody against a T cell leukemia biopsy of human origin) (sc-71113, Santa Cruz).

Techniques: Transgenic Assay, Over Expression, Reverse Transcription Polymerase Chain Reaction, Western Blot, Expressing, Staining